Western blot analysis of extracts from HeLa cells, untransfected or transfected with control (-) or Aurora B (+) siRNA, demonstrating a reduction in Histone H3 phosphorylation. Aurora B was detected using Aurora B monoclonal antibody, phospho-Histone H3 was detected using Phospho-Histone H3 (Ser10) (6G3) Monoclonal Antibody #9706, and total Histone H3 was detected using Histone H3 Antibody #9715.
Western blot analysis of extracts from HeLa cells, untransfected or transfected with control (-) or targeted (+) siRNA. Aurora B was detected using Aurora B monoclonal antibody, Aurora A was detected using Aurora A/AIK Antibody #3092. The Aurora B monoclonal antibody confirms silencing of Aurora B expression, and the Aurora A/AIK Antibody is used to control for loading and specificity of Aurora B siRNA.
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.